| 1. | And we have also studied its expression in protoryotes and the conserved regions of insect chitinase genes in different insects pests
Sds page电泳显示，在50th附近没有特异蛋白带的出现，此结果表明外源基因没有得到表达。 |
| 2. | An about 550 bps and an about 700 bps fragment could be seen after the vector pqe80l / dhfr / hpd - 3 was digested by bamh i + kpn i and by bamh i + hindiii r
经w亡sternblot证实，在大小分别约为3ikd和26kd的位置有两条清楚的蛋白带。 |
| 3. | Meanwhile , the results show that sp - d , sp - j , sp - z and sp - c are relatively tolerant to lincomycin , but the other 6 strains of meterials are not
7kd的一条蛋白带，而直线形藻丝体即mo则缺失此带，表明此53 7l蛋白与螺旋藻的形态变异相关。 |
| 4. | The purity was detected by sds - page , and only igg ' s heavy chain and light chain protein tope were showed up . the activity of igg was tested by technique of immune credeschs gold
用sds - page进行igg的纯度检验，电泳结果出现两条蛋白带，是igg的重链和轻链，说明纯化的程度较高。 |
| 5. | But the mutation of this gene has not been found in those samples by pcr - sscp and this indicated that this gene might carry out its function through up - regulation or down - regulation
将表达产物破包涵体后经glutathinonesepharose4b柱亲和层析分离， sds page显示纯化的蛋白质为一分子量60kd的单一蛋白带。 |
| 6. | The expressed protein was purified and used to generate antibodies in rabbits . western blot analysis of extracts of hasnpv - infected hzaml cells revealed a specific protein of 43 kda from 48 h to 96 h p . i .
被hasnpv感染的hzami细胞的westernblot分析发现，从感染后48hyg96h检测到一条43kda大小的蛋白带。 |
| 7. | After sequencing the vectors were transformed into e . coli dh5 a and recombinant fusion protein was expressed via induction of iptg . fusion protein coded by carboxyl terminal gene sequence was purified through glutathione agarose column
经sds page分析，在相对分于质量（ mr ）为74000和43000处，各有1条特异的蛋白带。 |
| 8. | Analysis of copper binding protein by sds - page , three main protein bands observed . the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc . 4
Sds page分析得到3条主要蛋白带，剪下这三条带进行胶内赖氨酸内切酶的消化，通过高效液相色谱分离肽段，选择性进行肽段的氨基酸序列测定。 |
| 9. | The target proteins with molecular weights of 35kd and 44kd were detected by sds - page . the 35kd target protein recovered from sds - page was used to immunize rabbit and the annsemm with high titer was obtained
Annfaf基因在大肠杆菌中以包含体的形式得到了高效表达， sds - page检测表明，分别获得44kd和35kd大小的蛋白带，与预期结果相同。 |
| 10. | On d2 after attachment , the protein bands attained 23 . after mating , the protein bands reached 26 and maintained the same until d4 after engorgement . these results meant that feeding and mating stimulated the biosynthesis of new protein
饥饿雌虫唾液腺有17条蛋白带：吸血后2d增加到23条；交配后达到26条，直到饱血后4d保持不变，吸血和交配能刺激新蛋白的合成。 |
