| 1. | We studied the application possibility of these two kinds of materials in cell culture engineering and biomedical engineering areas
第二章中，继续探讨血清培养情况下细胞的选择性脱附。 |
| 2. | The culture results of the porcine ear skin fibroblasts was good by using dmem / f12 containing 20 % fetal bovine serum
在培养条件方面选取dmem f12培养液+ 20胎牛血清培养猪耳皮肤成纤维细胞，效果较好。 |
| 3. | Experimental condition optimization study on inducing mouse embryonic stem cells differentiation into insulin producing cells by serum - free culture in vitro
无血清培养法诱导小鼠胚胎干细胞分化为胰岛素分泌细胞的实验条件优化选择 |
| 4. | Effects of nuclear factor - b decoy oligodeoxynucleotide on the function of human umbilical artery smooth muscle cells induced by umbilical sera in preeclumpsia
顺式诱骗寡脱氧核苷酸对子痫前期患者脐血清培养的脐动脉平滑肌细胞功能的影响 |
| 5. | Objective to investigate the culturing technique for conjunctival epithelial cells and to evaluate the facibility of serum - free culture system on culturing conjunctival epithelial explants
摘要目的研究结膜上皮细胞体外培养的方法，并进一步探索无血清培养系统用于结膜上皮细胞培养的可行性。 |
| 6. | Methods rabbit conjunctival epithelial tissues were explanted in serum - free system the morphology , gowth , proliferation and differentiation of outgrow cells were compared with those in serum - containing medium
方法分别用含胎牛血清培养液和不含血清培养液进行组织块培养，观察两种培养方法在细胞形态、细胞生长情况和增殖及细胞分化方面的差异。 |
| 7. | Conclusion this is a serum - free culture system for developing a conjunctival epithelial explants with improved purify and proliferative , which are important for the basic research and tissue engineer of conjunctival epithelial cells
结论无血清培养系统的组织块培养法可为结膜上皮细胞的疾病、药理毒理研究及培养移植的结膜上皮细胞提供更为安全有效的细胞模型。 |
| 8. | They used this method to improve the surface lubricity , antithrombogenicity , antibacterial , nettability and cell growth activity of the biomaterials , get a series of photo - chemical immobilized biomaterials which had good biocompatibility
接着在这一新材料表面对鼠成纤维细胞( sto )进行无血清培养，培养2小时后，在低于lcst的温度下对sto细胞进行选择性脱附。 |
| 9. | Results the epithelial cells cultured in serum - free medium not only demonstrated a significant increase in proliferative , colony - forming efficiency and cell generations , but also have the normal structural and functional properties than those in serum - containing medium
结果无血清培养系统中结膜上皮细胞的增殖、克隆形成及细胞传代能力均较含血清培养系统高，而且具备结膜上皮细胞全部组织结构及功能特点。 |
| 10. | Methods : in cultured lung explants without serum , the lipid component synthesis of pulmonary surfactant was evaluated in [ 3h ] - choline incorporation ; mrna content of phosphocholine cytidylyltransferase ( cct ) in lung explants was investigated in rt - pcr ; the changes of the ultrastructure of the at ii cells were observed with electron microscope ; the expression of nmdar1 subtype was observed in immunohistochemistry staining ; nitric oxide synthase ( nos ) activity , nitric oxide ( no ) content , superoxide dismutase ( sod ) level , malondialdehyde ( mda ) content and lactae dehydroase ( ldh ) level were determined by biochemistry methods . results : 1 . influence of glutamate on synthesis of the lipid component of pulmonary surfactant ? with l - arginine , glu inhibited [ 3h ] - choline incorporation with good dose - dependence and time - dependence ; ( 2 ) mrna content of cct of the glu treatment groups was decreased ; ( 3 ) glu increases the release of ldh in cultured lung explants ; ( dwith electron microscope histochemistry , glu induced the changes of the ultrastruture of at ii iv cells
方法：采用成年大鼠肺组织无血清培养，运用[ ~ 3h ] -胆碱掺入法测定ps主要脂质磷脂酰胆碱( pc )合成量； rt - pcr扩增检测肺组织中pc合成限速酶磷酸胆碱二胞苷酰基转移酶( cct ) mrna含量；透射电子显微镜法观察肺泡型上皮细胞和ps系统超微结构的变化；免疫组织化学染色检测glu的受体nmdar1亚单位的表达；生化测定肺组织乳酸脱氢酶( ldh )释放量和肺组织匀浆中一氧化氮合酶( nos )活性、一氧化氮( no )生成量、超氧化物歧化酶( sod )水平以及丙二醛( mda )含量。 |
