| 1. | An improved alkaline lysis adaptive to extracting plasmid dna of bacillus
提取的改良碱裂解法 |
| 2. | Methods : plasmid profile analysis was used
方法：碱裂解法。 |
| 3. | The plasmids of the three bacteria were identified , the results showed m8 had n ' t plasmid , the plasmid of m6 and ml3 were about 20kb
用碱裂解法提取质粒dna ，结果发现： m8没有检测到质粒， m6和m13的质粒大小都为20kb左右。 |
| 4. | The presence of plasmid in bacillus was isolated by improved alkaline lysis . only one plasmid pbl29 was detected in bacillus iicheniformis29
本文采用改良碱裂解法对30株益生芽孢杆菌进行质粒抽提，仅从一株地衣芽孢杆菌b |
| 5. | Rubber and rubber products - determination of the composition of vulcanizates and uncured compounds by thermogravimetry - acrylonitrile - butadiene and halobutyl rubbers
橡胶和橡胶制品.利用热裂解法测定硫化和未硫化化合物的成分.丙烯晴丁二烯和丁基卤化橡胶 |
| 6. | Two phases atomizing nozzle and parallel board electrostatic precipitator were designed . ti02 powders were synthesized by plasma spraying with ethanol solution of butyl titanate as liquid feedstock on the optimized parameters
对等离子喷涂工艺进行了优化，使用喷雾裂解法，以钛酸丁酯的乙醇溶液作为喷涂原料，成功的合成了纳米tio _ 2颗粒。 |
| 7. | The plasmid extraction from wild strains needs an appropriate method . first , lysis by alkali was used to extract plasmid from strain pseudomonas xn - 1 . but the expected result had not been got , and a lot of cracked plasmid dna fragments were got only
对于野生菌株的质粒提取，需要选择合适的提取方法，我们首先采用了细菌细胞质粒提取常用的碱裂解法，但是未取得理想的效果，只得到了许多破碎的质粒dna片段。 |
| 8. | Chapter 2 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) by cdna macroarray analysis plasmids were extracted from all the clones in the subtracted cdna libraries with alkali lysis method , then the inserted fragments in plasmids were amplified by pcr
2用cdna微阵列筛选中华绒螯蟹卵巢发育相关基因将差减cdna文库中的所有克隆用碱裂解法提取质粒，然后以提取的质粒为模板，用pcr方法扩增插入片段。 |
| 9. | So some methods suitable to large plasmid extraction , including lysis by sds and a method from a literature , were used to try to extract the large plasmid from the strain cell . the lysis reactions in these two methods are gentle , so the large plasmid cannot be injured in the lysis process , opposite to lysis by alkali . it would be helpful to keep the integrality of the large plasmid during the extraction
因此我们采用了适合于大质粒提取的sds法，和文献中应用于硝基苯降解性质粒的提取方法，来尝试对菌株细胞进行质粒提取，这两种方法裂解反应温和，不会像碱裂解法那样，在裂解过程中损坏质粒，可以实现质粒提取的完整性。 |
| 10. | Cell morphology was similar no matter what method was used . percentage of live cells was significantly higher in erythrocyte splitting method that was ( 91 4 ) % than in centrifugation method that was ( 83 5 ) % . proliferative ability of mscs seperated by erythrocyte splitting method at 2 to 6 generation was much higher than by another method . the maximal cell number was 4 . 67 vs 4 . 10 times that of the initial cell number at cell inoculation
红细胞裂解法较密度梯度离心法细胞贴壁较慢， 36小时才可见零星细胞贴壁，两种方法细胞形态相似。活细胞百分比，红细胞裂解法为（ gi士4 ） ，密度梯度离心治（ 83士5 ） ，有明显差异。传代培养： 2 6代范围内，红细胞裂解法较梯度离心法细胞增殖更旺盛（分别是按种时细胞数的4 |
