It is used as the bait to screen azospirillum brasilense sp7 genomic plasmid library which was constructed by fusing 0 . 5 - 3kb fragments of a . brasilense sp7 genomic dna to the dna - activation domain in the pgad plasmid vectors Brasilensesp7的nifa全序列构建在pgbd - c2载体上，得到重组质粒pgbd - nifa ，以其为诱饵，筛选sp7质粒基因组文库(构建在pgad系列质粒上) 。
