| 1. | Analyzing the characteristics of start codon in prokaryote and yeast genomes
原核生物和酵母基因组中起始密码的特征分析 |
| 2. | Chain initiating codon
链起始密码子 |
| 3. | In order that bar gene efficiently transcript and translate in eukaryote , the sequence next to atg was modified by pcr
为了目的基因在真核生物中有效的翻译和表达，通过pcr方法对bar基因起始密码子atg旁侧序列进行改造。 |
| 4. | ( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed
( 2 )在翻译水平上通过pcr扩增的方式在t - pa基因起始密码子处添加了植物翻译起始共有序列aaca ，构建了植物表达载体pbet 。 |
| 5. | A late baculoviral transcription initiation motif ataag was found 65 nt upstream of the putative translational start site and a polyadenylation signal aataaa was identified 14 nt downstream of the taa stop codon
在其起始密码子上游- 65nt发现杆状病毒晚期启动子转录起始信号ataag ，在其终止密码子下游- 14nt发现polya加尾信号aataaa 。 |
| 6. | The sequence encodes an open reading frame of 518 amino acids . there is a transit peptide of 74 amino acids in the n terminal of the putative amino acids sequence coded by the cdna
该序列长为1758hp ，起始密码子位于84 86hp ，终止密码子为1658 166fop ，开放阅读框长为1557hp ，编码58个氨基酸序列，其中n末端含有74个氨基酸的转运肽。 |
| 7. | 5 ' race analysis indicated that ha 122 transcripts start predominantly in the consensus major late transcription initiation motif rtaag around 47nt upstream of the putative translation start codon with a minor start at position - 89
5 ’ race ( rapidamplificationofcdnaend )分析结果表明ha122基因的转录主要从翻译起始密码子上游第47个碱基起始，该处有一个晚期转录信号taag 。 |
| 8. | By using the method of genome walking , two fragments were cloned from genome walker library after the semi - nested pcr . the result of sequencing the bigger fragment showed that this 706bp sequence contained the atg start codon
通过半巢式的基因步移法，经二轮pcr后，从genomewalker文库分离到2个片段，较大的片段测序表明该706bp序列含有编码ast的起始密码子atg ，其上游序列为启动子序列。 |
| 9. | A late transcriptional motif , ataag , was found at - 50nt upstream of the translational start codon atg , while two tata box was located at - 112nt and - 189nt upstream of atg . a typical poly ( a ) signal was found at 12nt downstream of the translational stop codon
在hel起始密码子atg上游50位有强晚期启动子转录起始信号ataag ，在? 112位和? 189位存在两个tatabox ，但未发现早期转录信号cagt 。 |
| 10. | After sequencing of 84 cdna clones and removing redundant cdnas , we obtained 36 cold - regulated unique cdna clones . 12 cdna clones were expected to be novel genes , because no sequence homology with any known sequences was found in genbank databases
全长基因ej175共有603bp ，对其可读阅读框架进行分析，从57 - 515位核苷酸的一段序列，包含了起始密码子和终止密码子，编码152个氨基酸的多肽。 |
