| 1. | Computational location of transcription start sites in prokaryotic genome based on sliding window
基于滑动窗口的原核转录起始位点计算定位方法 |
| 2. | By artificially changing a to c at - 137 bp site upstream from transcription start point of cloned promoter , two site - mutation promoters , ipms ( 603 bp ) and ipm1 ( 900 bp ) were created
通过pcr方法对克隆的两个启动子进行定点突变，使转录起始位点上游- 137bp处a突变为c ，得到两个突变启动子( ipml 、 ipms ) 。 |
| 3. | Activity of each construct was determined . the basal promoter was located at about 60bp up stream of the transcription initiation site . it contains a tata box at - 33bp which is required for the transcription initiation
基因的基本启动子元件位于转录起始位点上游约60bp ，其中含有一个位于- 33bp处的tatabox ，它对于转录起始起着重要作用。 |
| 4. | 900 bp promoter - directed gus expression was highly induced by sa and bth , while the 603 bp promoter , whether mutated or not , did not respond to sa and bth induction , which indicated that the element in response to sa and bth lied among 575 ~ 872 bp from transcription start site
全长900bp启动子能够应答sa和bth的诱导，而603bp长的启动子无论突变与否对sa和bth均无应答，证明sa和bth的应答区域在克隆启动子的转录起始位点上游575 872bp之间。 |
| 5. | In order to use the responding ability to the inducers of pr - la promoter , two fragments , ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank . sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence . its transcription start site , tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter
根据pr - 1a基因的报道序列，设计两对引物，以烟草基因组为模板，通过pcr扩增得到900bp ( ipl )和603bp ( ips )两个目标片段，序列测定表明克隆的启动子与报道序列具有99的同源性，转录起始位点、 tatabox及保守序列tgac与报道序列均完全相同。 |
| 6. | The entire halcs gene is located on a megaplasmid and contains a 849 - bp open reading frame that encodes a polypeptide of 283 amino acids . the promoter is typically haloarchaeal , but the start site of transcription is only seven bases from the 5 ' end of the initiator aug codon , making the halcs transcript another example of a " leadless transcript " in the haloarchaea
它的一29一24位置上是一“毛幻人” box序列，为典型的嗜盐古菌启动子;它的转录起始位点距离“ atg ”只有7个核普酸，也是典型的嗜盐古菌的“卜adless腼script ’ ’ 。 |
| 7. | It is suggested that 538bp sequence of ast gene 5 " end had been cloned after the 138bp fragment was linked up with the 706bp fragment . the analysis of 538bp sequence with the software of promoter prediction indicated that there maybe exist four transcriptional initiator sites , one caat - box and two gc - boxes
将该片段与706bp的片段对接后，表明克隆到了ast基因的上游启动子的538bp序列，通过promoterpredict软件进行启动子的分析，显示该序列存在可能的四个转录起始位点，一个caat框和两个gc框。 |
| 8. | In the part i , the sequence of the cloned promoter osg6b " was first analyzed . osg6b " had a whole length of 1688 bp and 98 % homology to known sequence of promoter osg6b . its transcriptional start point , tata boxes and the consensus sequences " tgtgg " conserved usually in anther - specific promoters were identical to those of reported osg6b
第一部分：对已克隆的启动子osg6b ’进行的序列分析表明， osg6b ’全长1688bp ，与报道的osg6b有98的同源性，两者在转录起始位点、 tatabox及其它花药特异性启动子共有的保守序列tgtgg完全相同。 |
