| 1. | Culture of rabbit osteoblasts digested by collagenase in dmem containing fetal bovine serum
含胎牛血清胶原酶消化法培养兔成骨细胞 |
| 2. | Conclusion the enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes
结论：酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。 |
| 3. | Methods using different techniques of collagenase digestion and discontinued density gradient centrifugation , the adult swine and rat islets were prepared
方法采用不同的胶原酶消化法及不连续密度梯度纯化法。 |
| 4. | The smg cell of rats were isolated and purified by pancreation digestin and then were cultured and subculfured in dmem with 20 % fetal bovine serum
应用胰酶消化法进行颌下腺细胞的分离、纯化及原代培养、传代培养。 |
| 5. | The viable cells after counting with trypan blue dye exclusion were then transferred to culture flask containing dmem medium in a density of 1 10 ^ 6
方法无菌条件下，从6月龄新西兰白兔的膝关节囊内剪取滑膜组织，采用组织块培养法和酶消化法分离滑膜细胞。 |
| 6. | At last , ctab - dna and sds - dna methods are used in this experiment . on the basis of optimizing experiment procedure and pcr system of the materials , the initial reversion transcription system and orthologus gene cloning technique are established
对这3种方法比较后，确定本实验采用ctab - dna酶消化法和sds - dna酶消化法提取白桦雄花芽组织的总rna 。 |
| 7. | In the current study , we tried to isolate liver progenitor cells from retrorsine - treated mouse . after long - term culture and purification , the pure epithelial cell population was established in cobblestone fashion with high nuclear - to - cytoplasm ratios
采用两步胶原酶消化法从损伤后的再生肝中分离细胞，经过长期的培养和不断的纯化，最终在体外建立了形态均一的连续细胞系。 |
| 8. | ( l ) . cultivation of dermal fibroblast : dermal fibroblasts were harvested by using enzyme digest technique from child foreskin after circumcision . 3 - 4 generation of cells were used as work cells . ( 2 ) cultivation of dermal fibroblast on collagen - chitosan spongy membrane
真皮成纤维细胞的分离培养：取包皮环切术后的幼儿包皮酶消化法分离培养真皮成纤维细胞并传代，以3 4代细胞作为工作细胞。 |
| 9. | Methods : vascular endothelial cells , smooth muscle cells and fibroblast are respectively isolated from human umbilical veins by enzyme digestion and tissue plant methods , subcultured , purified and identified , etc . phase - contrast and electron microscopy was used to analyze the cells morphological characteristics
方法：分别采用酶消化法和组织块法培养血管内皮细胞、血管平滑肌细胞及成纤维细胞，并进行三种细胞的传代、纯化、鉴定以及形态学观察。 |
| 10. | Since there are more things in the bud tissue of the male flower , such as phenolic compounds , polysacchrides , proteins and some other secondary metabolites , three methods used to isolate rna are tested in this assay , which are the enzyme digesting methods of ctab , ctab - dna and sds - dna
克服了常规方法和试剂盒无法提取出富含酚类化合物、多糖和一些尚无法确定的次级代谢产物的白桦花芽组织rna的障碍，提出了3种白桦雄花芽组织rna的提取方法： ctab 、 ctab - dna酶消化法和sds - dna酶消化法。 |
