| 1. | 3 . bioinformatics analysis revealed that bt - r3 protein is a member of cadherin superfamily
生物信息学研究发现： bt - r3受体蛋白是钙粘蛋白超级家族中的一员。 |
| 2. | There are two kinds of receptor proteins of bt toxic protein in insect body , aminopeptidase receptors and cadherin - like receptors
靶标昆虫中bt受体蛋白主要有两类：氨肽酶和类钙粘蛋白。 |
| 3. | A leucine zipper pattern is located in the membrane proximal extracellular domain ( mped ) of bt - r3 protein . this is a very important character of cadherin
另外，它还有1个亮氨酸拉链序列，位于胞外的近膜区( mpr ) ，这是钙粘蛋白的重要特征之一。 |
| 4. | Other domains of the extracellular part of bt - r3 protein are 11 cadherin repeated domains , which mainly consist of p stands that are linked by loop structure , turn residues and random coil
其余的胞外区域形成以折叠为主的11个钙粘蛋白重复结构域。这些d折叠又由loop结构、转角和无规卷曲连结在一起。 |
| 5. | Expression system renders products that are free of post - translational modifications , it is likely that the binding of crylab toxin to bt - r3 protein via protein - protein interaction and does not require glycosylation
且该受体蛋白与cry1ab的结合部位位于bt - r3受体蛋白的胞外结构域。 bt毒蛋白与钙粘蛋白受体的结合与受体是否糖基化无关。 |
| 6. | 2 . the molecular weights of the recombinant protein of the cadherin repeated domain 4 , 5 , 6 of bt - r3 were about 42kda and 45kda ( with his tag ) respectively . both expressed products could not specifically bind to bt toxic protein crylab
表达得到了bt - r3受体的第4 ， 5 ， 6个钙粘蛋白结构域重组蛋白，其分子量约为42kda和45kda (带histag ) ，均不能有效地结合bt毒蛋白cry1ab 。 |
| 7. | It was reported that cadherin - like receptors are most likely the main receptors of bt toxic protein . bt - r3 , a gene of 5551 , is a newly cloned cadherin - like receptor gene and its orf ( open reading frame ) encodes 1715 amino acids
Bt - r3受体基因是吴志平博士在其博士论文研究中新克隆到的钙粘蛋白类受体基因，它的cdna序列全长5551bp ，编码1715个氨基酸残基组成的bt - r3前体蛋白。 |
| 8. | The cadherin repeated domains of bt - r3 protein share high homology with the cadherin domains from african clawed frog ( xenopus laevis ) and e - cadherin ( epithelial ) from mouse ( mus musculus ) . so , the 3rd structure of the cadherin repeated domains of bt - r3 protein should be similar to these domains
Bt - r3受体蛋白的钙粘蛋白结构域分别与非洲爪蟾( xenopuslaevis )的钙粘蛋白胞外结构域和小鼠( musmusculus )的上皮钙粘蛋白胞外结构域有很高的同源性。 |
| 9. | Catenin binds to the cytoplasmic tail of a transmembrane cell adhesion molecule - epithelial cadherin ( e - cadherin ) in epithelial cells and forms an adhesion complex [ e - cadherin - p - catenin ( or y - catenin ) - cc - catenin ] ( cadherin - catenin - complex , ccc ) , this complex play an important role in maintaining epithelial cell adhesion . some catenins such as - , - and p120ctn catenins are also crucial in cell signaling , they play an important role in cell proliferation and cell apoptosis
在上皮细胞内，连环蛋白均能与跨膜细胞粘附分子?上皮钙粘蛋白( e - cadherin )的胞内肽段结合而形成了由上皮钙粘蛋白- -连环蛋白( -连环蛋白) - -连环蛋白组成的粘附连接体( adherensjunctions , ajs ) ，这种复合体对维持细胞正常的粘附功能是必需的。 |
| 10. | P120ctn is a more newly found catenin , studies revealed that it is also an important molecule in e - cadherin mediated cell - cell adhesion , and phosphorylated tyrosine in p120ctn were evidenced . translocation of p120ctn occurred in cancer cells and tissues . evidences showed that there were a key relationship between p120ctn translocation and tumoregenesis
P120 ~ ( ctn )是发现得较晚的一种连环蛋白，研究发现它也参与上皮钙粘蛋白介导的细胞粘附过程并且不可缺少，同时p120 ~ ( ctn )也和-连环蛋白一样易于酪氨酸残基磷酸化，磷酸化后的p120 ~ ( ctn )也发生转位，并与肿瘤的发生发展有密切关系。 |
