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Home > english-chinese > "链可变区" in Chinese

Chinese translation for "链可变区"

gly4ser

Related Translations:
可变:  variable; changeable◇可变比 variable ratio; 可变成本 direct costs; variable costs; 可变电容 variable capacity; variable capacitance; 可变电容器 variable condenser; variable capacitor; variodenser; adjusta
可变滤波器:  variable filter
可变点:  running pointvariable point
可变利益:  variable profit
可变汇率:  flexible rate
可变喉管:  variable venturi
可变耦合:  variable coupling
可变切除:  variable muting
可变减速:  varial deceleration
可变翼:  variable geometry; swing-wing◇可变翼飞机 adjustable [variable] wing plane; swing-wing
Example Sentences:
1.This is important in attempting to use antibodies as anti - tumor reagents , for example
基因为日本血吸虫单克隆抗独特型抗体np30轻链和重链可变区基固。
2.The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library . one of positive scfv clones , named pcsal , was selected with phage - elisa after panning and screening by bull sperm three times . scfv fragment , amplified from pcsa1 , was ligated to pmd18 - t vector for sequencing analysis
取阳性重组噬菌体抗体克隆株pcsa1 , pcr扩增其scfv基因,筛选重组子进行序列测定,发现其序列符合小鼠抗体基因的一般特征,并且与几株抗磷酸胆碱的抗体重链和轻链可变区序列的同源性达80以上;推测pcsa1scfv针对的抗原是磷酸胆碱类物质。
3.Thus , immunologists have sought smaller molecules with the antigen - recognition capability of antibodies . the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable . it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues
二、日本血吸虫单克隆杭独特型抗体np30的单链狐( scf )的构建、表达及对balbic小鼠诱导保护性作用研究l 、日本血吸虫单克隆杭独特型抗体np0的单链抗体归cfv )的构建、表达通过pcr方法体外扩增并经测序验证的重链、轻链可变区( vh 、 vl )基因先后重组入原核表达质粒ptha90相应的位点上,中间通过一连接肽( gly在er ) 。
4.Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library . the products were cloned into puc19 vector and their sequences were analysed . the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region , with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以从噬菌体抗体库中筛选获得的抗hbsag的fab抗体基因为模板,分别扩增出其轻、重链可变区( v _ l 、 v _ h )基因,通过重组pcr方法将轻、重链可变区基因用连接肽( gly _ 4ser ) _ 3的编码序列连接,并引入前导肽编码序列,构建具有l - v _ h - linker - v _ l结构的单链抗体基因。
5.In the present study , the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis . total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies . after obtained using rpas system , vh and vl genes were used to assemble scfv gene fragment with a linker primer
应用重组噬菌体抗体库技术,从分泌小鼠抗牛精子sp18抗体的杂交瘤细胞系中分离总rna ,克隆抗体重链和轻链可变区基因,加入连接肽引物( linkerprimer )组装成单链抗体scfv ( singlechainfragmentvariable )基因并用rs引物进行扩增, sfi 、 not酶切,回收后与pcantab5e载体相连,转化e . colitg1宿主菌,构建单链抗体文库。
6.Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2 . construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr . the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e . co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重组噬菌体抗体库的构建及鉴定从培养的mg _ 7杂交瘤细胞中提取并分离mrna ,反转录成cdna ;利用pcr分别扩增mg _ 7单抗的重链及轻链可变区基因,并通过? dna连接子将二者连接起来形成mg _ 7单链抗体基因;将mg _ 7单链抗体基因插入pcantab5e ;将连接产物转化感受态tg1大肠杆菌,制备细菌形式的mg _ 7重组噬菌体抗体库;通过菌落计数和限制性酶切分析( ecor和hind )评估mg _ 7重组噬菌体抗体库的容量和重组率。
Similar Words:
"链菌素-生物素蛋白-过氧化物酶连接法" Chinese translation, "链卡子" Chinese translation, "链开始" Chinese translation, "链开始指示符" Chinese translation, "链可变菌素" Chinese translation, "链空间" Chinese translation, "链孔浮筒" Chinese translation, "链孔盖" Chinese translation, "链孔肘板" Chinese translation, "链控舱口盖" Chinese translation