Chinese translation for "高效价"

hbig

Related Translations:

高效消毒剂:  high effect disinfectants

高效暴雨:  high efficiency storm

高效节能:  energy-efficient

高效政府:  an honest, diligent, pragmatic and efficient government

高效钻进:  efficient drilling

高效农业:  high-efficiency agriculture

高效处理:  high-activity processing

高效过滤:  high-rate filtration

高效筛选:  high-throughput screening

高效措施:  expedient measure

Example Sentences:

	1.	Analysis of high - titer cold agglutinins causing cross - matching test difficult 高效价冷凝集素致交叉配血困难分析
	2.	A case report of discrepancy between forward typing and reverse typing due to high ti - ter cold auto - antibody 高效价自身冷抗体致血型正反定型不符1例
	3.	Antibody is one of the most important reagents commonly used in immunological cell chemistry technology . actually nowadays highly specific and sensitive antibodies are of vital importance to any molecular biological experiment 抗体是免疫细胞化学技术的首要试剂，制备具有高特异性和敏感性的高效价抗体在细胞生物学研究方面具有重要意义。
	4.	In order to get the soluble recombinant eo protein and inspect the protein expression status convinently , the egfp and eo gene were ligated into baculovirus transfer vector . with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna , and plaque purification in the posttransfection procedure , the pure recombinant baculovirus were harvested , which infected the sf9 cells for amplifying to generate a p - l stock . . in the meantime , the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus . the pi - stock from a pure plaque was used to generate a high liter p - 2 stock , which was determined in liter as 1 . 14 107pfu / ml by performing a plaque assay . when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression , the strong fluorescence was obeserved on the day 3 of postinfection 此外，为了得到可溶性重组eo蛋白并便于观察重组蛋白的表达情况，我们将egfp基因与eo基因相连插入昆虫杆状病毒转移载体中，与线性杆状病毒dna共转染sf9细胞后通过噬斑纯化得到纯的重组杆状病毒，将其感染sf9细胞制备p1种子液，同时用荧光显微镜观察绿色荧光蛋白的表达情况剔除表达效果差的重组杆状病毒。再用p1种子液感染sf9细胞制备高效价的p2种子液。通过病毒液的梯度稀释和噬斑测定，确定p2种子液的病毒滴度达1 . 14 10 ~ 7pfu ml 。
	5.	The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2 , ha order to prepare ndrg2 antibody , the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector . after induced by iptg , the fusion proteins were expressed in e . coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization , rabbits produced high titer antiserum against ndrg2 . then antisemm was absorbed using ndrg2 antigen immobilized on nc filters , the purified product of antiserum shows high special to ndrg2 protein , and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach 为制备高效价的ndrgz抗体，分别构建了prset a雌、 pgex4t d唾仓和pgex4tl七三种原核重组表达质粒，并在大肠杆菌中诱导表达出相应的融合蛋白；用全长gstjqdrgz蛋白免疫兔，然后用gst ndrgz人和gstjqdrgze片段加强免疫，经免疫得到了较高效价的兔抗人ndrz多克隆抗血清，利用固定于硝酸纤维素膜上的ndrgz抗原亲和吸附纯化抗血清，提高了ndrgz抗体的特异性；并对包涵体形式表达的6his ndrgz进行初步的分离纯化。
	6.	Then using ecbp21 antibody and immunogold transmission electron microscopy method , we studied the subcellular localization of ecbp21 . the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica . these results indicated that ecbp21 mainly localized in cell wall , which provide a direct evidence of the extracellular existence of ecbp21 . furthermore , using ecbp21 antibody and immunohistochemical method , we studied the organic specially distribution of ecbp21 , the results indicated that ecbp21 distributed in all organize , but it distributed more in leave n flower rachis than in leafstalk and root 首先，构建了ecbp21表达载体，诱导了重组蛋白的表达，并通过胶回收法获得了大量纯化重组ecbp21蛋白，制备了高效价、高特异性抗体；随后，利用ecbp21抗体，结合免疫胶体金电镜定位技术进行了ecbp21亚细胞定位研究，结果显示：在白芷愈伤组织细胞和花序轴细胞中金颗粒主要分布在细胞壁区域，而在细胞内未发现或仅有少量金颗粒分布，表明ecbp21蛋白主要定位于细胞壁区域，这为细胞外cambp ( ecbp21 )的胞外存在提供了直接证据：进一步，利用ecbp21抗体，通过免疫组织化学分析研究了ecbp21组织特异性分布状况，结果表明ecbp21在白芷各组织中均有分布，但在叶、花、花序轴中分布较多，而在叶柄、根中分布较少。
	7.	On the other hand , the expression is repressed by the proto - oncogenes n - myc and c - myc , and in some cases the expression is also repressed by testosterone or dihydrotestosterone ( androgen ) at mrna level infering from all the references that can be obtained , the functions of ndrg family may be involved in cellular differentiation events > maintaining the balance of cell redox potential ( in oxidative stress condition or biological conversion process ) , or counteracting cell malignant transformation , and so on 综合己有的文献，推测ndrg家族可能参与细胞生长分化、维持细胞氧化还原电势平衡（氧化应激或生物转化） 、阻止肿瘤细胞恶化等。因此，研究其在细胞中的具体功能以及可能的信号通路将具有非常重要的意义。深人研究ndrgz在体内的组织细胞和亚细胞水平分布特点及其功能，需要高效价和高特异性的ndrgz抗体。
	8.	Anti - melatonin monoclonal antibodies of higher titer , affinity and good sensitivity were obtained by coupling mt to bovine serum albumin with formaldehyde and by immunizing mice with multifocal intra - dermal injections . we obtained 6 strains of hybridoma , all of them secreting specific antibodies to mt , we apply antibodies to determinate free mt inhuman serun with group - selective immunoassay technique . an inhibition curve for mt was obtained in the range of 50pg to30ng , and 1 . 4ng of mt inhibited the value of the assay by half . we evaluate the specificity of antibodies by determination of cross - reactivity of several analogues , the moabs recognized mt but 通过将mt用甲醛作连结剂连结到牛血清白蛋白上sa采用皮下多点注射兔疫小鼠得到了高效价，高亲和力，较好特异性的抗mt单克隆抗体，最后获得了5株单克隆细胞株，都能分泌针对mt的特异性抗体，建立了选择性基团免疫分析法，用制备的抗体测定了人血清中mt的含量，作了mt的抑制标准曲线，其抑制范围从50pg ? 30ng ，半抑制量为1

Similar Words：

"高效活化修复晚霜" Chinese translation, "高效活能修护霜" Chinese translation, "高效活性热力面膜" Chinese translation, "高效货币" Chinese translation, "高效挤出机" Chinese translation, "高效价,低酸度" Chinese translation, "高效价,高滴度" Chinese translation, "高效价冷凝集素综合征" Chinese translation, "高效价噬菌体液" Chinese translation, "高效价铜绿假单胞菌免疫球蛋白" Chinese translation