English translation for "dna重组"
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- dna recombinant
dna recombination
Related Translations:
重组: bprdefragmentationreassemblereassemblyrecombinant dna technologyrecombination: heteroduplex and mismatch repair in vitrorecombination: strand transferasesrecombinerecomposereconstructionre dna电泳: dna electrophoresis
- Example Sentences:
| 1. | Mistakes in the dna reconstruction process ,
Dna重组过程中的错误 | | 2. | Scbi 536 basic principles of animal behavior
Dna重组工程原理 | | 3. | The committee recommended that recombination dna work at princeton be considered in this same light
委员会建议用同样的观点来考虑在普林斯顿的dna重组工作。 | | 4. | This work is the analysis of metabolic pathway and designing rational genetic modification to optimize cellular properties by using principle of molecular biology
本研究根据代谢工程原理系统分析了细胞代谢网络，并利用dna重组技术合理设计细胞代谢途径及其遗传修饰，进而完成细胞特性改造。 | | 5. | In 1987 , the notion of a splicing system was introduced by tom head in [ 3 ] as a mathematical model of restriction enzyme digestion and subsequence religation in the recombination of dna molecules
1987年， tomhead发表了一篇论文，引入拼接系统( splicingsystem )的概念作为限制酶与dna (脱氧核糖核酸)作用、进行dna重组的数学模型。 | | 6. | The expressive gene fragment was 333bp long . analyses of the tcr y fragment shpwed that it contained three epitopes . it was showed that specific anti - idiotypic antibody could be found since four weeks after the first immunization and came to the climate on the sixth week . the antibody titers of the same time were higher in group pcdna3 / tcr y - il - 2 than in group pcdna3 / tcr y ( po . 01 ) . the highest antibody titer was 1 : 640 in the group pcdna3 / tcr y - il - 2 , whereas the highest was 1 : 160 in the group pcdna3 / tcr y . there were mrna expression in skeletal muscle cell could be found in group pcdnas / tcr y and group pcdna3 / tcr y - il - 2 on fifth day after inoculation
用tcryvi独特型dna重组载体作为疫苗免疫小鼠，结果表明pcdna3汀cry组及pcdna3厅cry ? il ? 2组小鼠1血肩中全部产生了特异性抗独特ffo抗体，抗体滴度在第4周开始增高，第6周时达到高峰。在同一取血时间， w儿1 ry一几一二组小鼠抗体滴度均高于pcdna3 tcry组（ p 0 1 ） 。 pcd a3 tcry组小鼠抗体滴度最高达1 ： 16小pc 。 | | 7. | However , its wide application in manufacture has always been restricted by such a question as low phytase - producing level of wild strains , so before the wild strains which can produce acidic phytase are widely used , their phytase activities must be improved by various means , in which using an efficient expression system to express heterogenous phytase is a main consideration
植酸酶主要存在于微生物和植物中，动物体内也有少量存在，但真正具有开发价值的主要是微生物产生的植酸酶，尤其是曲霉产生的酸性植酸酶。国外早在1991年就成功地利用dna重组技术获得第一株酸性植酸酶的工程菌，并已投入商品化生产。 | | 8. | Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点，设计合成一对特异引物，在pt7 - 7 - rpf4表达质粒的基础上，应用聚合酶链式反应( pcr )对其cdna进行改造，通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ，用快速pcr检测法、 dna测序分析，鉴定重组hpf4表达质粒的正确性。 | | 9. | The pil - 6 cdna fragment was inserted into pgex - 1 ? t plasmid to construct the expression plasmid pgpil - 6 , the recombinant plasmid was digested by bamh i and pst i to identify whether the pil - 6 cdna fragment was inserted into the plasmid in correct orientation , the pgpil - 6 was transformed into e . coli dh5 ? competent cells
用dna重组技术将已克隆的猪il ? 6cdna片断插入pgex ? 1 t质粒，构建了猪il - 6基因的原核表达质粒pgpil - 6 ，转化大肠杆菌，以bamhi酶切筛选、 psti酶切鉴定转化子，获得了含740bp插入克隆基因正确的重组子。 | | 10. | By recombinant dna techniques , the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb . the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e . coli dh5a and induced with iptg . sds - page analysis showed an induced expression product band about 72ku , which correspond to the sizes of vp2 , reported in the literature
利用dna重组技术，将其结构蛋白vp2基因亚克隆至原核表达载体pproex - htb ， iptg诱导后成功表达出与预期大小相符的约72ku的融合蛋白，光密度扫描对表达产物进行初步定量，表明表达产物约占菌体总蛋白的14 。 |
- Similar Words：
- "dna肿瘤病毒" English translation, "dna肿瘤簿" English translation, "dna重复" English translation, "dna重复序列" English translation, "dna重新退火蛋白" English translation, "dna重组技术" English translation, "dna重组技术,脱氧核糖核酸重组技术" English translation, "dna重组作用" English translation, "dna转核苷酰酶" English translation, "dna转录酶" English translation
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