English translation for "dna重组技术"
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- recombinant dna technique
Related Translations:
重组: bprdefragmentationreassemblereassemblyrecombinant dna technologyrecombination: heteroduplex and mismatch repair in vitrorecombination: strand transferasesrecombinerecomposereconstructionre dna电泳: dna electrophoresis
- Example Sentences:
| 1. | This work is the analysis of metabolic pathway and designing rational genetic modification to optimize cellular properties by using principle of molecular biology
本研究根据代谢工程原理系统分析了细胞代谢网络，并利用dna重组技术合理设计细胞代谢途径及其遗传修饰，进而完成细胞特性改造。 | | 2. | However , its wide application in manufacture has always been restricted by such a question as low phytase - producing level of wild strains , so before the wild strains which can produce acidic phytase are widely used , their phytase activities must be improved by various means , in which using an efficient expression system to express heterogenous phytase is a main consideration
植酸酶主要存在于微生物和植物中，动物体内也有少量存在，但真正具有开发价值的主要是微生物产生的植酸酶，尤其是曲霉产生的酸性植酸酶。国外早在1991年就成功地利用dna重组技术获得第一株酸性植酸酶的工程菌，并已投入商品化生产。 | | 3. | Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点，设计合成一对特异引物，在pt7 - 7 - rpf4表达质粒的基础上，应用聚合酶链式反应( pcr )对其cdna进行改造，通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ，用快速pcr检测法、 dna测序分析，鉴定重组hpf4表达质粒的正确性。 | | 4. | The pil - 6 cdna fragment was inserted into pgex - 1 ? t plasmid to construct the expression plasmid pgpil - 6 , the recombinant plasmid was digested by bamh i and pst i to identify whether the pil - 6 cdna fragment was inserted into the plasmid in correct orientation , the pgpil - 6 was transformed into e . coli dh5 ? competent cells
用dna重组技术将已克隆的猪il ? 6cdna片断插入pgex ? 1 t质粒，构建了猪il - 6基因的原核表达质粒pgpil - 6 ，转化大肠杆菌，以bamhi酶切筛选、 psti酶切鉴定转化子，获得了含740bp插入克隆基因正确的重组子。 | | 5. | By recombinant dna techniques , the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb . the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e . coli dh5a and induced with iptg . sds - page analysis showed an induced expression product band about 72ku , which correspond to the sizes of vp2 , reported in the literature
利用dna重组技术，将其结构蛋白vp2基因亚克隆至原核表达载体pproex - htb ， iptg诱导后成功表达出与预期大小相符的约72ku的融合蛋白，光密度扫描对表达产物进行初步定量，表明表达产物约占菌体总蛋白的14 。 | | 6. | With the development of dna recombination technology , there are also some reports about the expression of recombined ifn protein by prokaryotic expression system , but the expressed proteins often consist in the form of inclusion body = the operations about extracting and purifying proteins are very complicated , biological activity of proteins are not high
随着dna重组技术的发展，国内外亦有利用原核表达系统表达重组ifn蛋白的报道，但表达的目的蛋白常以包涵体形式存在，提取纯化操作步骤复杂、蛋白活性不高。 | | 7. | The results were as following : 1 . construction and identifcation of recombinant plant expression vector pbi ! 2i - th by dna recombination technology , sweet protein thaumatin gene was cloned into plant expression vector pbii2i . recombinant plasmid pbii2i - th was constructed successfully by enzyme cutting and electrophoresis
甜蛋白thaumatin基因植物表达质粒pbi _ ( 121 ) - th的构建与鉴定利用dna重组技术，将植物甜蛋白thaumatin基因克隆至植物表达载体pbi _ ( 121 )中，通过酶切、电泳，鉴定thaumatin基因已成功构建到植物表达质粒pbi _ ( 121 )中。 |
- Similar Words：
- "dna肿瘤簿" English translation, "dna重复" English translation, "dna重复序列" English translation, "dna重新退火蛋白" English translation, "dna重组" English translation, "dna重组技术,脱氧核糖核酸重组技术" English translation, "dna重组作用" English translation, "dna转核苷酰酶" English translation, "dna转录酶" English translation, "dna转录物" English translation
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