| 1. | Immunocytochemical properties of raphe nucleus neurons in rat brain
大鼠脑中缝核的免疫细胞化学特性研究 |
| 2. | Electron microscopy and immunocytochemical method were used to evaluate the cultivated cells
结果：培养的周细胞贴壁生长，胞体肥大，呈不规则状、有较多突起。 |
| 3. | An immunocytochemical method for identification of bursaphelenchus xylophilus antigen on the surface of pinewood section
疫木切面上松材线虫抗原的免疫学检测方法 |
| 4. | The tumor was subtotally resected ; pathologic and immunocytochemical findings were compatible with embryonal carcinoma
手术后病人出现尿崩症，持续高烧不退及水脑证。 |
| 5. | The application of immunocytochemical technique in the bone marrow sections in patients with myelodysplastic syndromes
免疫组织化学技术在骨髓增生异常综合征骨髓病理中的应用 |
| 6. | The cultivated cells showed positive reaction for - smooth muscle actin ( - sma ) antibody , and negative reaction for factor antibody with immunocytochemical assay
细胞表面有微绒毛，胞质内有丰富的细胞器，无因子小体；免疫细胞化学显示细胞呈平滑肌肌动蛋白( - sma )阳性，因子相关抗原阴性。 |
| 7. | The primary cell propagated 8 times and were small fusiform or triangle and smaller than hepatocytes with larger nucleus and less plasma ; it was 88 . 2 % in the growing period of 4 ( superscript th ) generation cells and the stains of alb and ck19 were positive in immunocytochemical
所获得的原代细胞共传8代，细胞呈小梭形或三角形，体积较小，胞核较大，而胞浆较少；第4代细胞中进入生长期的细胞约占88 . 2 % ；免疫细胞化学染色显示细胞胞质中alb 、 ck19染色阳性。 |
| 8. | The behavioral observation of nociceptive responses were carried out immediately after formalin administration by examining the total time used for licking and biting towards the injected hindpaw by rats , and then , one hour later after formalin injection , rats were killed for immunocytochemical procedures for fos expression in the dorsal horn
R ，或mgi叭:的拮抗剂c尸ccoet / mpe尸。以单纯注射formalin侧背角的fos表达( fli神经元)作为对照，对同一脊髓双侧背角的fl工进行观察、记数并进行比较和统计学处理。 |
| 9. | In the present experiment studies , an acute traumatic model of lateral cortical impact was employed to study expressive changes of microtubule associated protein - 2 ( map - 2 ) , cyclooxygenase - 2 ( cox - 2 ) , glial cell line - derived neurotrophic factor ( gdnf ) , caspase - 3 mrna and protein after brain injury in rats . immunocytochemical staining , western blotting , nucleic acid in situ hybridization with an oligonucleotide probe and computer image analysis were used to detect the dynamic changes of map - 2 mrna , cox - 2 mrna , gdnf mrna , and caspase - 3 mrna in the cortex after moderate traumatic brain injury ( tbi )
本实验从自行设计大鼠脑损伤落体打击器开始，先行建立了一个便于观察和施加处理因素、控制性好、重复性好的动物模型，选用30g击锤从25cm高处下落，冲击应力d为355 . 09kpa ，打击大鼠右顶部，造成中等程度的闭合性脑损伤，从病理形态学、组织超微结构观察及微管相关蛋白- 2 ( microtubuleassociatedprotein2 ， map - 2 ) 、环氧合酶- 2 ( cyclooxygenase2 ， cox - 2 ) 、胶质源性神经营养因子( glialcellline - derivedneutrophicfactor ， gdnf ) 、 caspase - 3基因及蛋白表达的时间性变化，详尽系统地阐述脑损伤后各指标变化的时间规律性及表达差异可能的形成机制。 |
| 10. | Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry . the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines , and compared with pgp inhibitor quinidine . the pgp expression of mcf - 7 adr was strongly positive , the positive rate was 15 % ; the pgp expression of mcf - 7 was negative , the positive rate was 1 . 8 % . ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115 . 2g ml and 207 . 6g ml respectively . ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129 . 8mol l 42 . 1g ml and 94 . 1mol l 30 . 5g ml respectively . tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7 , but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly . immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively . tea polyphenol is not only an anti - tumor agent , but also a multidrug resistant modulator similar as quinidine . tea polyphenol is advantageous for its little toxicity in tumor treatment
用免疫组化法和流式细胞仪对肿瘤细胞系mcf - 7和mcf - 7 adr的p -糖蛋白表达水平进行定性定量研究。用噻唑蓝比色法mtt研究茶多酚的细胞毒性及其对耐药性的逆转作用，并与pgp抑制剂奎尼定进行了比较。免疫组化法检测p -糖蛋白表达水平， mcf - 7 adr呈强阳性，而mcf - 7呈阴性流式细胞仪定量检测结果mcf - 7 adr细胞系细胞阳性率为15 % ， mcf - 7细胞系细胞阳性率为1 . 8 % 。 |
