| 1. | We used immunocytochemistry to explore the expression of il - 2r
免疫细胞化学反应：检测ilzr的表达； 5 |
| 2. | From the point of morphology and immunocytochemistry , it is proved that it is reasonable to analysis the follicular steroidogenesis of the rana quadranus by using the two - cell type model
从形态学和免疫细胞化学的角度证明，用两类细胞模型解释卵巢类固醇激素的合成与分泌是合理的。 |
| 3. | Two hours later , according to the protocol of the sabc kit of boster company , an immunocytochemistry was done to examine whether there is neuron - specific proteins in cytoplasm
5小时后用含2 dmso的诱导液处理5个小时。取诱导10小时后的细胞，参照博士德公司的sabc试剂盒操作方法进行免疫细胞化学实验。 |
| 4. | There were significant difference between psd group and the two control groups ( p < 0 . 05 ) , no significant difference between the two control groups . 4 . immunocytochemistry : the c - jun positive cell nuclei appeared brownish granules
正常对照组和快眼动睡眠剥夺对照组海马各区均未见明显的c jun阳性信号表达。 |
| 5. | Result the result of the first part : 1 . immunocytochemistry reveals that breast cancer cells of t47d and mda - mb - 231 have positive reaction . positive particles position cell membrance and cell plasma respectively
结果：第一部分结果：天津医科大学硕士学位论文中文摘要1 .免疫组化显示: t47d阳性，阳性反应物定位于胞膜和胞浆。 |
| 6. | P - liquid scintillation counting was used for analysis of erk and immunocytochemistry was used for the analysis of c - jun . all the data were analyzed with statistical treatment . the results were showed as follows : 1
分别做p液闪计数观察erk的活性表达，做westernblot观察jnk的表达量变化，免疫组化观察cjun阳性信号的表达，并进行统计学处理，结果如下： 1 |
| 7. | The fetal liver stem cells were isolated by collagenase digestion , gravity sedimentation and density gradient centrifugation , identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition
采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞，通过免疫细胞化学方法对其进行初步鉴定，以及应用流式细胞仪等对其生长状况进行评估。 |
| 8. | Two days later ( that is , these cells had been cultured for ten days in vitro from isolation ) these cells were collected and immunocytochemistry was performed by anti - nestin to identify nscs and by anti - brdu to evaluate nscs " proliferating capability
3 . 4ap25一35和ap25一35 +雌激素作用后细胞增殖能力检测对照组神经干细胞brdu阳性细胞率约为50 % ;雌激素保护组及ap毒性组brdu几乎未见阳性细胞。 |
| 9. | M ethods we successfully expanded human embryonic brain - derived nsc into spheres with mitogens . the nsc were identified by immunocytochemistry method , brdu labeling and cell cloning were used to observe the proliferation ability of nsc . pdgf x t3 were separately used to induce the differetiation of nsc
方法：本实验以胎龄为10 - 12周的人大脑皮质为材料，在体外成功诱导扩增nsc ，用免疫细胞化学方法鉴定nsc ，用brdu标记和细胞克隆分析观察了nsc的增殖能力。 |
| 10. | In the laboratory experiment part , human peripheral blood , cultured cells and icr mice were study objects . the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test . the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test . the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry , western blot and rt - pcr
流行病学结果显示长期小剂量辐射接触与染色体不分离呈正相关，为进一步在细胞遗传学和分子生物学方面研究小剂量电离辐射与染色体不分离关系及其机制，本课题第二部分以外周血、培养细胞、 icr小鼠为研究对象，用外周血染色体计数和单细胞受精卵染色体计数的方法研究小剂量辐射和拓扑异构酶复旦大学2000级博士生学位论文11a抑制剂及其二者的协同效应对有丝分裂和减数分裂染色体不分离的影响，用免疫细胞化学染色、 westernblot 、 rt pcr等方法研究了电离辐射引起拓扑异构酶a表达变化。 |
