| 1. | Were amplified and assembled into a single gene using a dna linker encoding a polypeptide of 15 amino acid residues gly
的基因片断，再经重组pcr将两片断由一编码gly |
| 2. | The team divided it on paper into 101 units ( the cassettes ) , each containing four or five genes
研究小组在纸上把它分为101个单位（基因片断） ，每个片段包含4到5个不等的基因。 |
| 3. | There is no cure for the progressive illness that robs people of their memory and mental ability but drug treatments may slow the early progression of the disorder
Apoe4基因片断常见于阿尔茨海默症患者，而普通人体内携带的则是apoe3基因。 |
| 4. | Polymerase chain reaction is a rapidly developing and widely used dna amplification technique , which is widely applied in life science and other related fields
聚合酶链式反应( polymerasechainreaction ，简称pcr )技术是发展很快、应用很广的体外扩增基因片断技术，在生命科学研究及诸多相关领域已经得到了广泛应用。 |
| 5. | The two researchers and their colleagues took a mixture of “ wild type ” mosquitoes and those that had had sm1 added to their genomes and fed them on mice infected with malarial parasites
这两位研究员和他们的同事一起将一些“野生”的蚊子和那些已经在其基因组中带有sm1基因片断的蚊子混合在一起，用感染疟原虫的老鼠来喂养他们。 |
| 6. | 2 . to construct the prokaryotic expression vector and the control vector . after sequenced , the target dna fragment was cloned into pqe - 80l vector together with the dna fragment encoding carrier protein dhfr
将自puc18 h d - 3回收的目的片段连同表达运载蛋白dhfr的基因片断一起连接于原核高效表达载体pqe - 80l ，经酶切鉴定，得到重组原核表达载体pqe - 80l dhfr h d - 3 。 |
| 7. | After the pbd i and pbd ii gene ligated with the expression vector pinpoint ? a - 3 , the recombinated plasmid ppd - 1 and ppd - 2 was transformed into jm109 strains , 4 positive clones were screened by restriction enzyme analysis , dna sequencing showed that two out of 4 positive clones inserted sequence of the constructed plasmid , which was the same as that of pbd - i and pbd - ii gene respectively , and its reading frame was correct , thus its could be used to express fusion protein
将pbd - 、 pbd -基因与表达载体pinpoint ~ ( tm ) xa - 3连结后获得的重组质粒ppd - 1 、 ppd - 2转化于大肠杆菌jm109中。经抽提质粒、酶切分析及pcr扩增，分别筛选到4个阳性克隆，将其中二个阳性克隆由测序分析，证实1个含pbd i基因片断， 1个含pbd 11基因片断，且阅读框正确，可用于融合蛋白表达。 |
| 8. | " although the simplest model for a cn affecting gene actiity is where the ariant is a deletion of a gene or part of a gene , we found examples where actiity is affected from a distance , " commented barbara stranger , first author and post - doctoral fellow at the wellcome trust sanger institute . " this may occur when the cn reduces the effectieness of a region that works to switch the genes on or off
变异拷贝数机制能通过改变特定基因的“剂量” （即复制后的拷贝数量） ，借助于裂解某个包含后续可翻译成蛋白质密码的基因的活性片断或借助于降解控制基因活性即类似开/关及在我们基因组中的调整开关的基因组中具有调控作用的基因片断来影响基因活性。 |
| 9. | By means of plant genetic engineering , foreign insects resistance gene can be transferred into plant cell . we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method . and obtained the transgenic mustard plants . the main results are as follows : 1 . isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves . the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers
本实验是利用植物基因工程获得抗虫的转基因芥菜植株，结果如下： 1豇豆胰蛋白酶抑制剂基因的分离分别提取豇豆种子、子叶及叶片的总rna ，逆转录成cdna 。以豇豆胰蛋白酶抑制剂基因两端的序列为引物，用rt - pcr的热启动方法从上述cdna中扩增出目的基因片断。 |
| 10. | Two strains of prrsv were isolated from the swine infected with prrsv in shangdong province and daqing area , in order to clarify the source and genetic background of porcine reproductive and respiratory syndrome virus ( prrsv ) from different parts of china , thus providing theoretic basis for the study of vaccine against it . the prrsv was cultured on mark - 145 cells for 5 ~ ~ 6 passages . when the cpe was obvious , the virus was harvested and purified
为了弄清我国不同地区prrsv的来源以及其遗传学背景，为疫苗学研究提供理论根据，本研究在ch - 1a株完整的基因组获得以后，从流行于我国山东( sd )和黑龙江大庆( dq )地区疑似prrs的猪体内分离到prrsv ，在mark - 145细胞上盲传5 6代，细胞出现明显病变以后，收获病毒液，然后提纯，提取全病毒rna ，经过反转录、 pcr扩增获得结构基因orf2 7的目的基因片断，然后与pmd - t载体连接，转化，得到阳性质粒后进行测序，并将其与ch - 1a株进行了比较分析，同时对这两个毒株的结构基因组的理化性质进行分析。 |
