| 1. | Importance of five genes presented in xenorhabdus nematophilus bp toxin gene cluster to its insecticidal activity
品系杀虫毒素基因簇中各基因与杀虫活性的关系 |
| 2. | And via the analysis of genetic array , 12 genes can be classified into 6 gene clusters in the chromosome . 2
经遗传排列分析发现，有12个基因在染色体上组成了6对基因簇( genecluster ) 。 |
| 3. | For gene manipulation such as heterologous expression of these huge gene clusters , new vectors and strategies are required
迄今为止，已经鉴定或克隆了50多个抗生素基因簇，其中许多基因簇大于40kb 。 |
| 4. | Virulence genes of pathogenic bacteria can be located on the chromosome where they may be organized as so - called pathogenicity island ( pai )
细菌毒力岛( pathogenicityisland ， pai )是指染色体上成簇排列的与细菌毒力密切相关的基因簇。 |
| 5. | The restriction map of phzl318 carrying the entire add gene cluster from s . avermitilis nrrl8165 was made . its two subclones phz2104 and phz2105 were introduced into s . lividans mutant zx1
制作了携带完整add基因簇的phz1318的限制内切酶图谱，根据酶谱进行亚克隆得到phz2104和phz2105 。 |
| 6. | Using pij903 bearing tsr gene as a vector , the two furthermost fragments of the cluster , 4 . okb and 1 . 5kb in size respectively , were inserted into it
Scp2 *的应用有两个主要的限制，一是必须有被克隆基因簇的完整而详细的遗传学信息，二是要求它在被克隆基因簇的来源菌株中不能复制。 |
| 7. | Analysis of the sequence revealed that adda resembled nifs of nitrogen - fixing bacteria and dnda . the entire add gene cluster showed 78 % identity to dnd gene cluster from s . lividans
同源性比较揭示add基因簇的adda基因与固氮酶基因nifs高度同源， add与变铅青链霉菌dnd核苷酸的同一性为78 。 |
| 8. | The chief aim of the present study is trying to extend the applications of yac and scp2 * , which have tremendous cloning capacity , for the above mentioned purpose . streptomyces sp . fr - 008 pks gene cluster ( c
本文对yac和scp2 *这类承载能力较大的载体系统在克隆fr - 008抗生素合成基因簇方面的利用进行了一系列载体改造和克隆策略方面的初步探索。 |
| 9. | The derivatives of phz2104 resulting from the disruption of the three putative orfs respectively by aada can not confer dnd phenotype on zx1 . using erase - a - base system , a series of exoiii progressive deletion mutants were prepared for sequencing of add gene cluster
将add基因簇亚克隆到测序载体pbluescript sk ( + )上，采用exo缺失突变法得到了66个单向递减缺失亚克隆，然后对这些克隆进行测序。 |
| 10. | In order to facilitate the study of biological function of add gene cluster , e . coli - s . avermitilis intragenus conjugation system was established . in addition , phz2114 for the replacement of the entire add gene cluster and phz2130 for disruption of adda were constructed
建立了除虫链霉菌的接合转移系统，并构建了用于置换全部基因簇的基因置换质粒phz2114和adda的基因中断质粒phz2130 ，为研究除虫链霉菌add基因簇的生物学功能奠定了基础。 |
