In the present study we investigated the effect of four weeks of treatment with a diet containing lactalbumin hydrolysate ( lah ; nestl , vevey , switzerland ) on the immune response of c3h / hen mice 摘要:本研究调查以水解乳白蛋白营养补充剂喂养小鼠四星期后对其免疫反应的影响。
2.
Mixing lactalbumin with either casein or soy protein in a 20 g protein / 100 g diet formula significantly enhanced the immune response in comparison to that of mice fed diets containing 20 % soy protein or casein 添加乳白蛋白到酪蛋白及大豆蛋白配方组的免疫反应较未添加者有显著增强。
3.
It have been reported that the 210 kb yeast artificial chromosome ( yac ) including the human a - lactalbumin gene could direct the transgene to express in a position - independent manner , therefore is an excellent expression vector to produce transgenic animal bioreactors 根据报道含完整人-乳白蛋白基因的yac载体能指导外源基因的乳腺特异性高效表达,是生产动物乳腺生物反应器的理想表达载体。
4.
The effect of 20 g / 100 g diet of lactalbumin ( l ) , casein ( c ) , soy ( s ) and wheat ( w ) protein on the immune responsiveness of c3h / hen mice has been investigated by measuring the humoral immune response to the t cell - independent antigen , tnp - ficoll 摘要:本研究测量体液对t细胞抗原的免疫反应,探讨不同的蛋白补充剂(包括乳白蛋白、酪蛋白、大豆蛋白及小麦蛋白)饲料( 20克蛋白/ 100克饲料)对小鼠免疫反应的效果。
5.
According to above consideration , experiments was carried out as below : first , targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology . secondly , the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa , then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene 因此,本试验首先扩增出整合在酵母基因组里的人血清白蛋白( hsa )基因作为目的基因,并将人血清白蛋白基因插入到一个含有人-乳白蛋白yac同源序列的重组型质粒载体,以构建整合型载体,再与另一个带筛选基因的质粒共转化入含人-乳白蛋白yac的酵母细胞体内。
6.
Finally 4 right clones was obtained from 864 transformants by pcr detection . sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac . furthermore , works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded 经pcr检测,从864个转化子中获得了4个阳性克隆,测序表明,人血清白蛋白基因已正确的导入到人-乳白蛋白基因yac的特定位点上,并获得了可进行细胞融合的重组yac载体。
7.
The plaque - forming cell response to sheep red blood cells was found to be enhanced in mice fed a formula diet containing 20 g lactalbumin / 100 g diet in comparison to mice fed equivalent formula diets of similar nutritional efficiency containing 20 g / 100 g diet of either casein , soy , wheat or corn protein , egg albumin , beef or fish protein , spirulina maxima , or scenedesmus protein , or purina mouse chow 摘要:本研究发现喂食特殊配方( 20克乳白蛋白/ 100克饲料)小鼠的溶血斑形成细胞对绵羊红血球的免疫反应增强,对照组喂食相等营养功效配方的酪蛋白、大豆、小麦或玉米蛋白、卵蛋白、牛肉或鱼肉蛋白、以及其它三种市售蛋白饲料。