| 1. | Mutants of subtilisin e
的突变体 |
| 2. | Enzymatic technology and analysis of enzymatic products of earthworm with subtilisin from bacillus subtilis as1
蚯蚓外源酶与内源酶酶解产物分析 |
| 3. | The sequence around these residues revealed that ap was a new member of the subtilisin family
该蛋白酶可以认为是枯草杆菌蛋白酶家族( subtilisin )的新成员。 |
| 4. | Based on the high homologous n - terminal amino acid sequence between ba - dfe and subtilisin bpn , the fragment encoding ba - dfe mature peptide was amplified from the total dna of b . amyloliquefaciens dc - 4 by pcr
根据ba - dfe的n -端序列与subtilisinbpn高度同源设计引物， pcr扩增出ba - dfe成熟肽编码区片段。 |
| 5. | In the same method , another pair of primers weredesigned in order to amplify subtilisin carlsbsrg from genomic dna of those bacillus strains by pcr . the cloning and sequencing of a fragment from bacillus licheniformis dy . are under investigation
又用扩增地衣芽孢杆菌碱性蛋白酶基因的引物在b . licheniformisdy的总dna中扩增到一条与预期大小相一致的片段，进一步实验仍在进行中。 |
| 6. | A pair of primers , based on the high homologous n - and c - terminal amino acids sequences of subtilisin of bacillus pumilus , were designed and used to amplify subtilisin gene from genomic dna of 4 different bacillus strains by polymerase chain reaction
Sds 、 edta和pmsf对酶活力有不同程度的抑制作用。以4株芽孢杆菌的总dna为模版，利用扩增短小芽孢杆菌碱性蛋白酶基因的引物对它们进行pcr扩增。 |
| 7. | Due to high homology of nucleotide between ba - dfe and subtilisin bpn , primers were designed and synthesized . the intact ba - dfe gene was amplified by pcr and cloned . the sequence analysis indicated that the ba - dfe gene has an open reading frame with 1146bp , which encodes 382 amino acid residues containing signal peptide , pro - peptide and mature peptide
序列分析显示，该片段的核昔酸序列中含有1146hp的开放阅读框，可编码382个氨基酸残基的ba dfe前体蛋白，包括30个氨基酸残基组成的信号肽、 77个氨基酸组成的前导肽和275个氨基酸残基组成的成熟肽。 |
| 8. | The gene encoding the mature peptide was cloned from the total rna of h rhossiliensis owvt1 by rtpcr . sequence analysis of the gene was described in this papel the amino acid sequence , as derived from the nucleotide sequence of a cdna clone , had high homology with other subtilisin - like serine protease of nematogenous fimgi
与其他丝氨酸蛋白酶基因序列比较表明，与其他线虫卵寄生性真菌如paecilomyceslilacinus 、 verticilliumchlamydosporium及m . anisopliaevar . anisopliae同源性较高，而与捕食线虫真菌arthrobotrysoligospora同源性较低( 45 ) 。 |
| 9. | Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae , extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates . the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents . a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用线虫诱导下owvt - 1菌株液体发酵，通过粗分级分离、离子交换层析和凝胶过滤层析分离提纯了一个分子量为31kda的丝氨酸蛋白酶，生物学测定表明其对大豆胞囊线虫二龄幼虫具有致死作用，同时测定了该酶理化特性，酶活力在75附近酶活力最高，随着ph的增加酶的稳定性升高，与胆碱酯酶具有相似的ph曲线，对特异性底物aape ( suc - ala - ala - pro - glu - pna )具有作用， ssi和ci - 2抑制该酶的活性。 |
