| 1. | Observed the infectious bronchitis virus shandong isolates by electron microscope
山东省传染性支气管炎病毒分离株的电镜观察 |
| 2. | Isolation and identification of nephropathogenic infectious bronchitis virus in chickens
鸡肾型传染性支气管炎病毒分离及鉴定 |
| 3. | Effects of reticuloendotheliosis virus infection on ifn - production in the splenocytes of spf chickens
利用基因重组技术将鸡传染性支气管炎病毒 |
| 4. | Molecular epidemiology of avian infectious bronchitis viruses isolated in guangxi by pcr and dna sequencing technique
及核苷酸序列分析技术对广西鸡传染性支气管炎病毒进行分子流行病学研究 |
| 5. | Infectious bronchitis ( ib ) caused by infectious bronchitis virus ( ibv ) is an acute and highly contagious disease in chickens
传染性支气管炎( ib )是由传染性支气管炎病毒( ibv )引起的一种急性高度接触性鸡传染病。 |
| 6. | The specificity of the nucleic acid probe was very strict . lt reacted positively with iltv dna only and it react negatively with the nuleic acid of ndv , bv and ibdv . the sensitivity of this kind of probe is very high . ilt could even detect 20pg ' s iltv dna
结果表明：该种核酸探针具有高度的特异性，它仅与iltv的dna呈现阳性反应，而与新城疫病毒、传染性法氏囊病病毒和传染性支气管炎病毒的核酸等均呈阴性反应。该种探针具有高度的敏感性，能够检测到20pg的iltv的dna 。 |
| 7. | Infectious bursal disease virus has been a great concern for the poultry industry for a long time , particularly for the past decade when its " re - emergence " in variant or highly virulent forms . in this study , two sets of primers ( pta and pts , ibda and ibds ) , flanking the hyper - variable region of vp2 gene , were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr . both of these assays can amplify all of 12 reference strains which including pathotypes cibdv , vvibdv and vibdv , but not the 5 negative reference pathogens of chicken
结果12个参考毒株均能扩增出约679bp的目的片段，而阴性对照的常见5种鸡病病原：鸡新城疫病毒( ndv ) 、鸡传染性支气管炎病毒( ibv ) 、鸡传染性贫血病毒( caiv ) 、大肠杆菌和多杀性巴氏杆菌均没有扩增到任何片段；应用建立的技术对疑似ibd的34份临床病料进行检测，并同时在基础rt - pcr扩增的片段内设计另一对引物进行nested - pcr 。 |
| 8. | Meanwhile , some transgenic plantlets and microtubers were transplanted into fields for reproduction and they survived . immunity assay with balb / c mice showed that expression product of transgenic potatoes with si gene of ibv had immunogenicity , and elisa antibody liter reached respectively 1 : 20 to 1 : 40 and 1 : 80 to 1 : 160 with doses of 0 . 5g and ig
Balb / c小鼠免疫试验表明， 1克转基因马铃薯剂量免疫小鼠后， 21天的抗体elisa效价达1 ： 80 ? 1 ： 160 ，说明传染性支气管炎病毒s1基因的转基因马铃薯表达产物具有良好的免疫原性，这为ib植物疫苗的制备奠定基础。 |
| 9. | The sequences of the structural protein genes and deduced a mino acid sequence of isolate lx4 were compared . by computer software , complete main structural genes sequence of ibv domestic strain and molecular characteristic genetic - variant analyses , and probably t cell and b cell epitopes of the main structural protein of infectious bronchitis virus were analyzed premently
通过计算机分析软件，对我国ibv地方流行毒株lx4的主要结构基因全序列、分子特征及遗传变异进行分析比较，并初步分析预测传染性支气管炎病毒主要结构蛋白上可能存在的t细胞和b细胞表位。 |
