| 1. | Molecular characterization of infectious bursal disease viruses - a review
传染性法氏囊病病毒分子生物学研究的一些进展 |
| 2. | Cloning and sequence analysis of full - length segment a of an infectious bursal disease virus strain
鸡传染性法氏囊病病毒的反向遗传研究 |
| 3. | Baculovirus - expressed system and research of infectious bursal disease virus gene expressed in a baculovirus recombinant
杆状病毒表达系统及其表达传染性法氏囊病病毒基因研究进展 |
| 4. | Expression of the infectious bursal disease virus polyprotein in vero cells using attenuated salmonella typhimurium as transgenic carrier
细胞中表达传染性法氏囊病病毒多聚蛋白基因 |
| 5. | Infectious bursal disease virus ( ibdv ) , the causative agent of infectious bursal disease ( ibd ) , causes immunosuppression in young chickens by destroying the precursors of b lymphocytes in the bursa of fabricius
传染性法氏囊病( ibd )是由传染性法氏囊病病毒( ibdv )引起的急性接触性传染病，是危害世界养禽业的主要传染病之一。 |
| 6. | The specificity of the nucleic acid probe was very strict . lt reacted positively with iltv dna only and it react negatively with the nuleic acid of ndv , bv and ibdv . the sensitivity of this kind of probe is very high . ilt could even detect 20pg ' s iltv dna
结果表明：该种核酸探针具有高度的特异性，它仅与iltv的dna呈现阳性反应，而与新城疫病毒、传染性法氏囊病病毒和传染性支气管炎病毒的核酸等均呈阴性反应。该种探针具有高度的敏感性，能够检测到20pg的iltv的dna 。 |
| 7. | The chickens and chicken embryos were inoculated with variant serotype isolate e of infectious bursal disease virus using cloacal , nosal routes or via the allantoic cavity route , and the histopathological features of the bursa of fabricius of the ibdv _ infected chickens at various intervals of time were systematically investigated
本试验全面而系统地观察了传染性法氏囊病病毒变异e株，通过泄殖腔、鼻腔和尿囊腔接种雏鸡和鸡胚后不同时间法氏囊的组织形态学变化。 |
| 8. | These results showed that the three isolates were infectious bursal disease virus . the full - length cdna of the genomic segment a of two viruses , one virulent field strain ibdv zj2000 and one attenuated strains ibdv jd1 , were amplified in a single step procedure by long - accurate reverse - transcription polymerase chain reaction ( la - pcr ) , cloned into pgem - t easy vector , and sequenced
分别以传染性法氏囊病病毒zj2000株(野毒株)和jd1株(弱毒株)基因组dsrna为模板，采用long - accuratert - pcr ( la - pcr )一步法扩增并克隆了两株病毒的基因组a节段全长cdna 。 |
| 9. | Three ibdv strains , designated as zj2000 , zj991 and zj992 , were isolated from bursa of fabricius of the sick chicken flocks in hangzhou , shenzhou , and ningbo of zhejiang province . the isolates could react with ibdv positive reference serum in the agar immune - diffusion test and caused 30 - 100 % mortality when passaged in chicken embryos . chickens inoculated with these isolates exhibited clinical and pathological signs typical of infectious bursal disease
从浙江省杭州、嵊州，宁波等地暴发疑似传染性法氏囊病的鸡群中采集法氏囊病料(编号分别为zj2000 、 zj991 、 zj992 ) ，分离病毒，经琼脂免疫扩散试验、动物回归试验、鸡胚回归试验和电镜观察等证实所分离的3株野毒是传染性法氏囊病病毒。 |
| 10. | Sequence and phylogenetic analysis of segement a of chinese infectious bursal disease virus ( ibdv ) hb - bp strain isolated from hebei province was studied in this thesis . the experiments as below were included : double - strand rna of viral genome was purified by licl gradient precipitation , 48 hours after bursa was harvested from chicken which had been inoculated with hb - bp strain . referred to the published sequence two primers were designed and synthesized
给4周龄spf雏鸡人工接种鸡传染性法氏囊病病毒hb - bp毒株， 48小时后采取法氏囊，用双licl分级沉淀法提纯全长基因组dsrna ，设计一对引物，通过rt - pcr方法进行了体外扩增，获得hb株a节段基因全长cdna 。 |
