| 1. | Nies - 90 microcystin producing algae strain genome library construction and one isolated gene analysis
构建用于相关基因筛选的微囊藻产毒株基因组文库 |
| 2. | A genomic dna library of atriplex centralasiatica iljin was constructed using lambda embl3 / bamh i as vector
为了得到badh基因的上游序列，我们以lambdaembl3 budl为载体，构建了盐生植物中亚滨茵的基因组文库。 |
| 3. | The me2155 genomic library was constructed using phz132 as the cosmid vector , and 10 positive clones were fished out using 0 . 9kb pcr product as probe
以phz132为载体，构建了mc ~ 2155的基因组文库。以0 . 9kb的扩增产物为探针从文库中钓出10个阳性克隆子。 |
| 4. | Strain sa - coo by southern hybridization . a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357 , a streptomyces - e . coli bifunctional vector carrying two cohesive sites
为了获得胆固醇氧化酶基因，以大肠杆菌-链霉菌双功能柯斯质粒phz1357为载体，构建了灰色链霉菌atcc14811的基因组文库。 |
| 5. | The plasmid dna was cut and dephosphorylated . the linearized plasmid dna was ligated to the pseudomonas sp . dna fragments and resulting recombinant plasmids were used to transform escherichia coli xll - blue
提取细菌总dna ，用限制性内切酶部分酶切后，回收2一6kbdna片段，与克隆载体puc18连接，转化大肠杆菌，构建了该菌株的基因组文库。 |
| 6. | It is used as the bait to screen azospirillum brasilense sp7 genomic plasmid library which was constructed by fusing 0 . 5 - 3kb fragments of a . brasilense sp7 genomic dna to the dna - activation domain in the pgad plasmid vectors
Brasilensesp7的nifa全序列构建在pgbd - c2载体上，得到重组质粒pgbd - nifa ，以其为诱饵，筛选sp7质粒基因组文库(构建在pgad系列质粒上) 。 |
| 7. | The two end - points were localized on a 4 . 3kb bamhl - ecor1 fragment of a cosmid 16c3 and a 1 . 2kb sail fragment of a cosmid 17g7 respectively . the deletion junction was localized on a 2 . 8kb bamhi fragment of the zx1 chromosome
两个端点分别定位在野生型菌株基因组文库粘粒16c3的4 . 3kb的bamh1 - ecor1片段上和17g7的1 . 2kb的sal片段上，缺失界点定位在zx1染色体上的一个2 . 8kbbamh片段上。 |
| 8. | Depending on the degenerate primers which were designed according to the conserved amino acids of glycine betaine secondary transporter , a fragment about 1 kb was obtained by pcr . the pcr fragment was purified and labeled with dig as a probe
将其总dna用sau3ai部分酶切后，收集4 15kb大小的酶切片段，克隆到用bamhi酶切的puc18中，构建halobacillussp . d8基因组文库，共获得约9000个重组质粒。 |
| 9. | In this paper several methods of developing microsatellite primers in plants were summarized : to search the database and find the primers which were delivered ; to transfer them among the different species ; to establish genomic library ; to develop ssr primer based on moleculars markers
摘要综述了开发植物微卫星引物的几种方法：搜寻核酸数据库和寻找已存在的微卫星引物；不同物种间共用；建立基因组文库；利用分子标记技术发展ssr引物。 |
| 10. | In this thesis , the fist cyanophage isolated in china was investigated with regard to the molecular biological characters . techniques for the large harvest and the electronic microscopy negative staining of the cyanophage were established . the genome library was constructed
论文对我国淡水中分离得到的一株蓝藻病毒(噬藻体)的某些分子生物学特性进行了研究，建立了其大量增殖与电镜染色的技术，构建了基因组文库，并对部分序列进行了测定与和分析比较。 |
