| 1. | The mediation of defense responses of ginseng cells to an elicitor from cell walls of colletotrichum lagerarium by plasma membrane nad h oxidases
拟南芥悬浮细胞及其愈伤组织对潮霉素的反应 |
| 2. | ( 2 ) the interest gene in pcar is under the control of acti which is the strong promoter in rice . the selectable marker gene is hyg gene
( 2 )表达载体pcar中目的基因由单子叶强启动子水稻act1启动子调控，选择标记基因为潮霉素抗性基因。 |
| 3. | Next , the researchers exposed the mushroom cells to hygromycin . the antibiotic kills all the normal cells , separating out those that hae been genetically altered for resistance
接着，研究人员用这些蘑菇细胞去接触潮霉素。这些抗生素可以杀死所有正常的细胞，分离出哪些能抵抗潮霉素的细胞。 |
| 4. | 3 . through the ladder experiment of selecting transformer concentration by hyg , the proper selecting transformer concentration is 20mg / l hyg to tomato cotyledons and 35mg / l hyg to tobacco discs
经潮霉素梯度筛选试验，最终确定番茄的适宜筛选浓度为20mg l ，烟草的适宜筛选浓度为35mg l ； 4 |
| 5. | The predicted bands were amplified from both to and ti generation transgenic plants by pcr , indicating that the hygromycin resistance gene ( hpt ) and svde gene were both transferred into tobacco
Pcr扩增潮霉素抗性基因hpt和svde基因结果显示，在‘转淬回植株t斤t ；代中都分别扩增出互okb和二 |
| 6. | 89 ) via agrobacterium mediation . pcr and southern blotting analysis showed that the cmo and badh genes were integrated into tobaccos " genome . betaine accumulation in transformed tobaccos was higher than that in control plants
89 ) ，获得卡那霉素抗性和潮霉素抗性植株， pcr和southern杂交均证明外源cmo和badh基因大连理工大学博士学位已整合到烟草基因组中。 |
| 7. | To create transgenic mushrooms , researchers attached a gene that confers resistance to hygromycin , an antibiotic , to circular pieces of bacterial dna called plasmids , which hae the ability to multiply within a bacterium known as agrobacterium
为了创造转基因蘑菇，研究人员需要把一种抵抗潮霉素（一种抗生素）的基因片段插入细菌dna的圆形片段（也叫质粒）中去，它将随着土壤杆菌复制而大量复制。 |
| 8. | When the seeds from to transgenic plants were cultured on medium supplemented with 50mg / l hygromycin , the ratio of germinated to non - germinated seeds is 3 : 1 , which is consisted with mendel ' s first law . southern blotting demonstrated that the svde gene had been integrated into the genome of tobacco plants
转基因植株的l代种了在潮霉素培养基上的萌发数与未萌发数的比值为3 ： l ，符合单基因的盂德尔分离规律。 |
| 9. | Four green plants were regenerated form resistant calli of wen . 6 derived form 1500 implanted embryos . no green plant was regenerated form calli of 200 non - transformed embryos . pcr assays of 4 green plants showed that two of them attained the expected size of amplified dna fragment ( 1500bp )
在含有20 - 60mg / l潮霉素的ms培养基上经诱导和继代培养后，获得了一批抗性愈伤组织，经过分化培养获得4株再生苗，对照的200枚幼胚未获得再生苗，对再生苗进行pcr检测。 |
| 10. | Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ) . nineteen individual kanamycin resistant plants were obtained . t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )
将ssvp和ssmapkk的全长cdna分别克隆入植物表达载体pcambia1300和prok中，导入根瘤农杆菌gv3101后，由花浸泡法进行拟南芥遗传转化，转化ssvp盐地碱蓬ssop和ssmapkk基因的克隆与功能鉴定的拟南芥在含潮霉素（ 25mg ）的ms培养基上筛选，获得t ；代转基因植株。 |
