TaqMan probes consist of a fluorophore covalently attached to the 5’-end of the oligonucleotide probe and a quencher at the 3’-end. La sonde TaqMan consiste en un fluorophore attaché de manière covalente à l'extrémité 5´ d'un oligonucléotide et en un désactivateur (quencher) à l'extrémité 3´ (Figure 1).
2.
In reality, only a fraction of the collisions with the quencher are effective at quenching, so the true quenching rate coefficient must be determined experimentally. En réalité, seule une fraction des collisions avec les molécules du désactivateur ont un effet sur la désactivation, de sorte qu'il faut en pratique mesurer expérimentalement le coefficient du taux de désactivation. ↑ Cf.
3.
The kinetics of this process follows the Stern–Volmer relationship: I f 0 I f = 1 + k q τ 0 ⋅ {\displaystyle {\frac {I_{f}^{0}}{I_{f}}}=1+k_{q}\tau _{0}\cdot } Where I f 0 {\displaystyle I_{f}^{0}} is the intensity, or rate of fluorescence, without a quencher, I f {\displaystyle I_{f}} is the intensity, or rate of fluorescence, with a quencher, k q {\displaystyle k_{q}} is the quencher rate coefficient, τ 0 {\displaystyle \tau _{0}} is the lifetime of the emissive excited state of A, without a quencher present and {\displaystyle } is the concentration of the quencher. La cinétique de ce mécanisme obéit à l’équation de Stern-Volmer : I f 0 I f = 1 + k q τ 0 ⋅
4.
The kinetics of this process follows the Stern–Volmer relationship: I f 0 I f = 1 + k q τ 0 ⋅ {\displaystyle {\frac {I_{f}^{0}}{I_{f}}}=1+k_{q}\tau _{0}\cdot } Where I f 0 {\displaystyle I_{f}^{0}} is the intensity, or rate of fluorescence, without a quencher, I f {\displaystyle I_{f}} is the intensity, or rate of fluorescence, with a quencher, k q {\displaystyle k_{q}} is the quencher rate coefficient, τ 0 {\displaystyle \tau _{0}} is the lifetime of the emissive excited state of A, without a quencher present and {\displaystyle } is the concentration of the quencher. La cinétique de ce mécanisme obéit à l’équation de Stern-Volmer : I f 0 I f = 1 + k q τ 0 ⋅
5.
The kinetics of this process follows the Stern–Volmer relationship: I f 0 I f = 1 + k q τ 0 ⋅ {\displaystyle {\frac {I_{f}^{0}}{I_{f}}}=1+k_{q}\tau _{0}\cdot } Where I f 0 {\displaystyle I_{f}^{0}} is the intensity, or rate of fluorescence, without a quencher, I f {\displaystyle I_{f}} is the intensity, or rate of fluorescence, with a quencher, k q {\displaystyle k_{q}} is the quencher rate coefficient, τ 0 {\displaystyle \tau _{0}} is the lifetime of the emissive excited state of A, without a quencher present and {\displaystyle } is the concentration of the quencher. La cinétique de ce mécanisme obéit à l’équation de Stern-Volmer : I f 0 I f = 1 + k q τ 0 ⋅
6.
The kinetics of this process follows the Stern–Volmer relationship: I f 0 I f = 1 + k q τ 0 ⋅ {\displaystyle {\frac {I_{f}^{0}}{I_{f}}}=1+k_{q}\tau _{0}\cdot } Where I f 0 {\displaystyle I_{f}^{0}} is the intensity, or rate of fluorescence, without a quencher, I f {\displaystyle I_{f}} is the intensity, or rate of fluorescence, with a quencher, k q {\displaystyle k_{q}} is the quencher rate coefficient, τ 0 {\displaystyle \tau _{0}} is the lifetime of the emissive excited state of A, without a quencher present and {\displaystyle } is the concentration of the quencher. La cinétique de ce mécanisme obéit à l’équation de Stern-Volmer : I f 0 I f = 1 + k q τ 0 ⋅
7.
The kinetics of this process follows the Stern–Volmer relationship: I f 0 I f = 1 + k q τ 0 ⋅ {\displaystyle {\frac {I_{f}^{0}}{I_{f}}}=1+k_{q}\tau _{0}\cdot } Where I f 0 {\displaystyle I_{f}^{0}} is the intensity, or rate of fluorescence, without a quencher, I f {\displaystyle I_{f}} is the intensity, or rate of fluorescence, with a quencher, k q {\displaystyle k_{q}} is the quencher rate coefficient, τ 0 {\displaystyle \tau _{0}} is the lifetime of the emissive excited state of A, without a quencher present and {\displaystyle } is the concentration of the quencher. La cinétique de ce mécanisme obéit à l’équation de Stern-Volmer : I f 0 I f = 1 + k q τ 0 ⋅
